All Candida species except for three (1.5 %) Candida kefyr strains were susceptible to amphotericin B. Only one (2.8 %) C. glabrata was resistant to fluconazole (MIC ≥ 64 μg/ml), and the others (97.2 %) exhibited dose-dependent susceptibility. All species, but C. glabrata strains, were susceptible to fluconazole Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study. (48%) was the most frequently isolated species, followed by Candida kruzei (16.1%), Candida glabrata (13.5%), Candida kefyr (7.4%), Candida parapsilosis (4.8%), Candida tropicalis (1.7%) and other species (8.5%). Resistance. Candida albicans was the most prevalent isolated species, followed by Candida glabrata, Candida tropicalis, Candida famata, Candida kefyr and Candida kuresi. The epidemiological cut off value (ECV) for all Candida species to amphotericin B was ≤0.25 μg except C. krusei (4 μg) Candida kefyr has been increasingly associated with bloodstream infections. This study reports on the identification and antifungal susceptibility pattern of 33 Candida isolates, identified biochemically as C. kefyr. One strain was finally identified as Candida sphaerica (Kluyveromyces lactis) by ITS sequencing
Antifungal susceptibility testing is a subject of interest in the field of medical mycology. The aim of the present study were the distributions and antifungal susceptibility patterns of various Candida species isolated from colonized and infected immunocompromised patients admitted to ten university hospitals in Iran. In totally, 846 Candida species were isolated from more than 4000 clinical. As expected, Candida krusei (seven strains) exhibited diminished in vitro susceptibility with MIC values for fluconazole of 8 to 128 micrograms ml-1 with a median of 64 micrograms ml-1. Some Candida kefyr strains seemed to be less susceptible against fluconazole which was indicated by a MIC90 of 64 micrograms ml-1 Site Antibiogram of Antifungal Susceptibility, by Candida Species and Agent Isolates collected in 2018 (Oregon) DRUG ABBREVIATIONS: AMB= amphotericin B; ANF = anidulafungin; CAS = caspofungin; FLU = fluconazole; 5FC = flucytosine; ISU = isavuconazole; ITC = itraconzaole; MFG = micafungin; PSC = posaconazole; Candida kefyr 1 0.06 0.03 0.03 0.
Antifungal susceptibility testing (AST) and genotypic heterogeneity among C. kefyr was also studied. Methods: Clinical C. kefyr isolates recovered from bloodstream and other specimens during 2011 to 2018 were retrospectively analyzed. All C. kefyr isolates were identified by CHROMagar Candida, Vitek2 and PCR amplification of rDNA Moreover, uncommon Candida spp. have emerged as causes of nosocomial bloodstream infections (BSIs) in studies of specific Candida spp. Those isolates commonly exhibit decreased in vitro susceptibility to antifungal agents (10 - 15). The epidemiology and clinical features of many uncommon Candida spp. BSIs have not been well characterized
Abstract In the central microbiology laboratory of Gazi University Hospital Candida kefyr was isolated from different clinical samples as 5.3% in 2016 and in 2017 this rate increased to 9.3% which was nearly two-fold and this has drawn our attention This species has been reported as an emerging pathogen [ 475 ]. This species may have been previously reported in the literature by the obsolete name of Candida pseudotropicalis. Reported infections include burn wounds [ 954 ], blood [ 1776 ], and vaginal [ 677 ]. FTL * in vitro susceptibility dat
Progress in antifungal susceptibility testing of Candida spp. by use of Clinical and Laboratory Standards Institute broth microdilution methods, 2010 to 2012. J Clin Microbiol . 2012 ; 50 : 2846 - 56 PubMed Overall, a total of five Candida species were resistant to fluconazole and four to voriconazole and among these species one each of C.parapsilosis, C.tropicalis, C.glabrata, C.lusitaniae, C.kefyr and three of C.albicans exhibited cross-resistance at least against two azoles Fahriye Eksi, Efgan Dogan Gayyurhan, Iclal Balci, In Vitro Susceptibility of Candida Species to Four Antifungal Agents Assessed by the Reference Broth Microdilution Method , The Scientific World Journal, vol. 2013, Article ID 236903, 6 pages, 2013. https://doi.org/10.1155/2013/23690
Candida kefyr has been increasingly associated with bloodstream infections. This study reports on the identification and antifungal susceptibility pattern of 33 Candida isolates, identified biochemically as C. kefyr.One strain was finally identified as Candida sphaerica (Kluyveromyces lactis) by ITS sequencing.Both species seem to be emerging pathogens and highly susceptible in vitro to. Kefyr clade consisted of Kluyveromyces marxianus (NRRL Y-8281), Candida kefyr teleomorph strain CBS 712, K. marxianus var. Kluyveromyces lactis strain NRRL Y-8279 and HC09C isolate of C. kefyr . Three isolates were classified into the Glabrata clade (HC01C, HC07C and HC10C), belonging to the colonized group and C. glabrata type (5478) with 100%.
.7 (18-73) Gender, male 44 (53.0) Ethnicity White 52 (62.7 PDF | Background: Candida kefyr, an emerging multidrug-resistant yeast, causes invasive candidiasis in susceptible patients. This study determined the... | Find, read and cite all the research you. Candida glabrata 80 13.5% Candida kefyr 44 7.4% Candida parapsilosis 29 4.8% Candida tropicalis 10 1.7% Others* 51 8.5% Total 595 100 Table 1. Distributions of Candida species isolates from patients 2005-2009. *Others include C. dublinensis 9, C.apicola 8, C. famata 4, C. zeylanoides 6, Cryptococcus neoformans 9, Trichosporon beigelii. 8. Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran:a five year study; Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran:a five year study. Authors . Badiee P. Alborzi, A. Affiliation CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Background and Objectives: The incidence of fungal infections in immunocompromised patients, especially by Candida species, has increased in recent years. This study was designed to identify Candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various clinical specimens
Candida spp. are the most common opportunistic mycosis worldwide. Although Candida albicans is the most common cause of urinary tract infections, the frequency of non-albicans Candida species is increasing with common use of antifungal in the prophylaxis and treatment. This may lead to difficulties in treatment. Antifungal tests should be applied with identification of species for effective. PDF | The activity of fluconazole, amphotericin B, caspofungin and micafungin was determined using XTT-based fungal damage assays against planktonic... | Find, read and cite all the research you. Among all isolates, 94% were susceptible to amphotericin B with MIC values of <1 μg/ml and all isolates were susceptible to caspofungin with MIC values of ≤0.5 μg/ml. Future studies are needed to define better treatment regimens for those patients who have fluconazole-resistant Candida urinary tract infections
The incidence of candidemia and the antifungal susceptibility patterns of the associated strains have not been extensively studied in Iran.The current study aimed to assess the microbial epidemiology of candidemia and the antifungal susceptibility profiles of Candida isolates.Out of 5141 blood culture specimens analyzed in Iran, 48 specimens. Hawkins JL, Baddour LM. Candida lusitaniae infections in the era of fluconazole availability. Clin Infect Dis 2003; 36:e14. Pfaller MA, Diekema DJ, Gibbs DL, et al. Geographic and temporal trends in isolation and antifungal susceptibility of Candida parapsilosis: a global assessment from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to. The application of in vitro susceptibility testing and the use of molecular methods have served to detect potentially resistant strains of Candida and to characterize completely the various mechanisms of resistance to fluconazole and other azoles in clinical isolates of Candida spp. (12, 44, 52, 64, 68, 78, 96, 110)
Species-specific susceptibility breakpoints for Candida kefyr have neither been published by the clinical laboratory institute (CLSI) nor by the European committee on antimicrobial susceptibility testing (EUCAST). Therefore we used the EUCAST breakpoints for Candida albicans for the interpretation of the MICs obtained with the Candida kefyr. The susceptibility of C. kefyr to amphotericin B appears to be quite low (4 of 10 isolates were susceptible at ≤1 μg/ml); however, it is uniformly susceptible to flucytosine, fluconazole, itraconazole, posaconazole, and voriconazole (Table 1). These findings are consistent with those of other investigators (3, 10, 19) As data on pediatric invasive candidiasis (IC) and the antifungal susceptibility pattern of associated isolates are scarce in Iran, this study aimed to determine species distribution and antifungal susceptibility profile of Candida species isolated from pediatric patients with suspected or documented IC The dairy yeast and facultative opportunistic fungal pathogen Kluyveromyces marxianus (syn.: Candida kefyr) -formerly Candida pseudotropicalis- is rarely associated with human infection and scarcely described as transient eye flora [12,13]. Here we present a case of keratomycosis caused by Kluyveromyces marxianus after DMEK . There is limited information about the distribution of Candida species and antifungal susceptibility in Turkey
mining the in vitro susceptibilities of 180 isolates of Candida spp. (50 C. albicans,50C. glabrata,10C. kefyr,20 C. krusei,10C. lusitaniae,20C. parapsilosis, and 20 C. tropicalis isolates) and 20 isolates of Cryptococcus neoformans. Overall, there was 100% agreement between the methods for amphotericin B, 95% agreement fo The study of Antifungal Susceptibility for Candida fungi was performed under the influence of unique human energy treatment. Read here for more details
The samples were seeded on Sabouraud agar supplemented with chloramphenicol. The 48 recovered isolates were phenotypically identified as 15 Candida famata, 13 Candida catenulata, 2 Candida intermedia, 1 Candida lusitaniae, 2 Candida guilliermondii, 1 Candida kefyr, 1 Candida amapae, 1 Candida krusei, 8 Trichosporon spp., and 4 Rhodotorula spp Candida tropicalis is considered the first or the second non-Candida albicans Candida (NCAC) species most frequently isolated from candidosis, mainly in patients admitted in intensive care units (ICUs), especially with cancer, requiring prolonged catheterization, or receiving broad-spectrum antibiotics. The proportion of candiduria and candidemia caused by C. tropicalis varies widely with. Reference strains or well characterized clinical isolates of Candida dubliniensis (CD36), Can-dida albicans (ATCC 90028), Candida glabrata (ATCC 90030), C. parapsilosis (ATCC 22019), C. orthopsilosis (ATCC 96139), Candida tropicalis (ATCC 750), Candida kefyr (Kw1609/11), Candida conglobata (Kw381/16), Candida utilis (Kw3642/15), Candida. The most important species which are considered pathogenic to humans are C. albicans, C. tropicalis, C. kruseii, C. glabrata, C. lusitaniae, Candida dubliniensis, Candida guilliermondii, Candida parapsilosis, Candida kefyr. (2) HiCrome Candida Differential Agar is a selective and a differential medium, which allows differentiation of Candida. Antifungal susceptibility testing of one isolate from each patient was determined by Etest. The 280 isolates forming pink-colored colonies on CHROMagar Candida, were identified by Vitek 2 as Candida haemulonii (n = 166), Candida utilis (n = 49), Candida kefyr (n = 45), Candida guilliermondii (n = 9), Candida famata (n = 6) and Candida conglobata (
The aim of this study was to determine the susceptibility of Candida isolates obtained from clinical specimens to the antifungal agents amphotericin B and fluconazole, which are frequently used in our clinical practice. C. glabrata, and C. kefyr isolates were inhibited by ≤1 μg mL−1 of amphotericin B, and fluconazole resistance was not. Prevalence and Fluconazole Susceptibility Profile of Candida spp. Clinical Isolates in a Brazilian Tertiary Hospital in Minas Gerais, Brazil Candida kefyr (n=2, 2.2%), Candida metapsilosis TABLE I Number (n) and percent (%) of strains collected from different clinical sources. The isolates were collecte Candida species distribution and antifungal susceptibility testing according to European Committee on Antimicrobial Susceptibility Testing and new vs. old Clinical and Laboratory Standards Institute clinical breakpoints: a 6-year prospective candidaemia survey from the fungal infection network of Switzerlan Susceptibility to antifungal agents of Candida species isolated from paediatric and adult patients with haematological diseases U. Nawrot,1 J. Nowicka,2 K. Juszczak3 and B. Gusin1 Departments of 1Microbiology, 2Haematology and 3Children Haematology and Oncology, Medical University of Wroclaw, Wroclaw, Poland Summary The susceptibility to six antifungals: amphotericin B (AMF), 5-ﬂuorocytosine. . to azoles that were determined with the three different meth-ods are shown in Tables 1 and 2. MICs for C. albicans, C. parapsilosis, C. tropicalis, and the other Candida (C. lusitaniae, C. dubliniensis, C. kefyr, and C. guilliermondii) isolates (n 128) were very low by all three.
The study of Antifungal Susceptibility for Candida fungi was performed under the influence of unique human energy treatment. The Candida kefyr exhibited a change in susceptibility against. Results. Candida albicans (48%) was the most frequently isolated species, followed by Candida kruzei (16.1%), Candida glabrata (13.5%), Candida kefyr (7.4%), Candida parapsilosis (4.8%), Candida tropicalis (1.7%) and other species (8.5%). Resistance varies depending on the species and the respective antifungal agents. Comparing the MIC90 for all the strains, the lower MIC90 was observed for. Among Candida species, Candida albicans (50%), C. tropicalis (25%), Candida glabrata (16.66%) and Candida kefyr (8.33%) were isolated. Antifungal drug susceptibility testing ericin B for all candida isolates. Resistance against fluconazole was present in 16 % of C.albicans isolates, 25% of C. tropicalis isolates
Among Candida species, Candida albicans (50%), C. tropicalis (25%), Candida glabrata (16.66%) and Candida kefyr (8.33%) were isolated. Antifungal drug susceptibility testing results shows 100% sensitivity to Amphotericin B for all candida isolates. Resistance against fluconazole was present in 16 % of C.albicans isolates, 25% of C. tropicalis. In order for Candida species to colonize the vagina, they must first adhere to vaginal epithelial cells. Candida albicans adheres to such cells in numbers significantly higher than those of C. tropicalis, C. krusei, and C. kefyr (17). This may explain the relative infrequency of the latter species causing vaginitis Candida Species Isolated from Various Clinical Samples and Their Susceptibility Patterns to Antifungals. Journal of Medical Microbiology and Infectious Diseases, 2013. JoMMID Journal. PDF. Download Free PDF. Free PDF. Download with Google Download with Facebook. or Candida kefyr strainY/16 was usedas control. The medium used was yeast nitrogen base glucose (YNBG) agar (HiMedia Laboratories, Mumbai, India). For susceptibilitytesting of azoles, 1.5% l- asparagine (HiMediaLaboratories, Mumbai, Prevalence and Antifungal Susceptibility of Candida albicans in Patna, India..
Systemic candidiasis has been on the rise in recent years due to the increasing number of patients with malignancies and use of immunosuppressants. The present study seeks to identify the distribution of Candida species isolated from malignant patients and determine in vitro antifungal susceptibility patterns of the isolates to promote their effective management.Blood and urine samples from. . Candida kefyr (teleomorph: Kluyveromyces marxianus) has been reported as a rare but potentially increasing cause of invasive candidiasis (IC) (1,- 3), especially in patients with hematologic malignancies (2, 4,- 6).Isolates of this species have been noted to develop reduced susceptibility to echinocandins, although the majority examined in population analyses remain. Molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis. Authors . Nejat Ziba, Abbasi Farahyar, Shirin Falahati, Mehraban khozani, Mahtab Ashrafi Hosseini, Aga, Fateme Faiazy, Azamsadat Ekhtiari, Masoom
Phylograms were rooted to The interest in a better characterization of the -psilosis group outgroup Candida kefyr ATCC 28838. relies not only on the epidemiology of C. parapsilosis but also The in vitro antifungal susceptibilities of the strains were on the suspected differences in the antifungal susceptibility tested according to a reference. In vitro susceptibility of invasive isolates of Candida spp. to anidulafungin, caspofungin, and micafungin: six years of global surveillance. J Clin Microbiol 2008; 46:150. Sun HY, Singh N. Characterisation of breakthrough invasive mycoses in echinocandin recipients: an evidence-based review Candida Kefyr Susceptibility If you suffer from through candidiasis, Candida Kefyr Susceptibility think about making modifications in. . Susceptibility testing procedures
Candida auris: an emerging species. C. auris is a multidrug-resistant pathogen increasing in incidence since its initial isolation and recognition from an ear swab in 2009 . Systemic candidiasis in critically ill patients on broad-spectrum antibiotics and/or antifungals has been the most common presentation Nenoff P (2016) In vitro susceptibility testing of yeasts to nystatin low minimum inhibitory concentrations suggest no indication of in vitro resistance of Candida albicans, Candida species or non-Candida yeast species to nystatin Clin Med Invest 2016 doi: 10.15761/CMI.1000113 Volume 1(3): 71-76 In each case, the yeast cell density was checked and confirme The frequency, antifungal susceptibility and enzymatic profiles of Candida species in cases of onychomycosis infection Author: Sav, Hafize, Microbial pathogenesis 2018 v.116 pp. 257-262 ISSN: 0882-4010 Subject: Candida albicans, Candida kefyr, Candida parapsilosis, Candida sake, Meyerozyma guilliermondii,. In Vitro Susceptibility of Candida Species Isolated from Cancer Patients to Some Antifungal Agents In Vitro Susceptibility of Candida Species Isolated from Cancer Patients to Some Antifungal Agents Özçelik , , B.; Çıtak , , S.; Cesur , , S.; Abbasoglu , , U.; Ιςlί , , F. 2004-06-01 00:00:00 Berrin Î Î¶Ï ÎµÎ Î Ï 1*, Sumru Â£itak 2 , Salih Cesur3, Ufuk Abbasoglu1 and Fikri.
As this method fails to detect amphotericin B-resistant Candida , the reported amphotericin B MICs were obtained by a more sensitive method based on use of Antibiotic Medium 3 in a agar-based testing format 614). Table 4. Interpretive breakpoints for antifungal susceptibility testing [Download PDF Yeast-like Fungi. Recently, several taxonomic rearrangements have been made and many well-known Candida species have been renamed and moved to other genera.. Notably Pichia kudriavzevii (formerly Candida krusei), Meyerozyma guilliermondii (formerly Candida guilliermondii), Clavispora lusitaniae (formerly Candida lusitaniae), Kluyveromyces marxianus (formerly Candida kefyr) and Wickerhamomyces.
Candida dubliniensis is a fungal opportunistic pathogen originally isolated from AIDS patients. It is also occasionally isolated from immunocompetent individuals. It is a dimorphic yeast  of the genus Candida, very closely related to Candida albicans but forming a distinct phylogenetic cluster in DNA fingerprinting.It is most commonly isolated from oral cavities, and is also. 51 Khadime Sylla et al.: Distribution of Candida Species and Their Susceptibility to Antifungal Drugs in Dakar, Senegal C. glabrata, C. parapsilosis, C. tropicalis, C. krusei and C. dubliniensis have been identified as main etiologic agent [4- 8]. The increased incidence and mortality related to invasive Candida infections (candidaemia) can be influenced b
Candida africana generated small green colonies on CHROMagar Candida and did not generate chlamydospores on CMA medium.Candida albicans strains grew at 42°C, while the C. africana isolates did not.Candida glabrata and C. kefyr (Kluyveromyces marxianus) produced dark pink colonies with pale edges and light violet colonies respectively.The clinical isolates with green-colored colonies on. Distribution of clinical isolates of Candida spp. and antifungal susceptibility of high biofilm-forming Candida isolates Gulcan Sahal and Isil Seyis Bilkay pink-salmon smooth colonies were deemed as Candida kefyr, while white-pale pink smooth colonies were considered as C. parapsilosis4,11,15 The collection included 4,283 strains of Candida albicans, 1,236 of Candida glabrata, 1,238 of Candida parapsilosis, 996 of Candida tropicalis, 270 of Candida krusei, 99 of Candida lusitaniae, 88 of Candida guilliermondii, and 61 of Candida kefyr. All isolates were obtained from blood or other normally sterile sites and represented the incident.
Candida glabrata has been reported in medical centers worldwide. Summary We checked all papers published along the last 10 years describing epidemiological, diagnostic, and clinical aspects of infections by MDR Candida spp., with emphasis on C. auris and C. glabrata spp. C. auris has been reported in 15 countries and multidrug resistance rates is usually above 30%. Horizontal transmission is a. Candida parapsilosis, and Candida tropicalis composed 75% of the isolates in our study, and the compatibility of these 3 species was found to be over 90% with all antifungals (AMB, FLC, VRC, CAS, and MFG). All Candida kefyr and Candida krusei were found to be 100% compatible with all antifungals. CA was excellent for AMB (98.4%), for CAS (94.5%) Please confirm that you would like to log out of Medscape. If you log out, you will be required to enter your username and password the next time you visit
(14.) MA Pfaller1, DJ Diekemal, RN Jones, SA Messer, RJ Hollis. Trends in Antifungal susceptibility of Candida species isolated from Pediatric and Adult patients with Blood stream infections: SENTRY Antimicrobial Surveillance Program. 1997-2000. glabrata 4 8% Candida guillermondi 3 6% Candida parapsilosis 1 2% Candida kefyr 3 6% Table 2. Thursday, June 6, 2013. Candida Krusei Susceptibility Candida Krusei Susceptibility Comparison of the VITEK 2 antifungal susceptibility system with Etest using clinical isolates of Candida species Author: Alfouzan, Wadha, Al-Enezi, Tahani, AlRoomi, Ebteehal, Sandhya, Vayalil, Chandy, Rachel, Khan, Zia Uddin Source: Revista iberoamericana de micología 2017 v.34 no.3 pp. 171-174 ISSN: 1130-1406 Subject
The aspects of the epidemiology of bloodstream Candida infections including clinical features, the causal agents, underlying conditions, and risk factors have not been well-defined in Iranian pediatric patients. The aim of this observational study was to identify uncommon Candida species isolated from blood and other normally sterile specimens of the neonates and children admitted to intensive. Candida albicans and non-Candida albicans differ significantly in their antifungal susceptibility pattern. Non- Candida albicans like Candida krusei are inherently resistant to azoles. Hence, species level identification with the in vitro antifungal susceptibility pattern is essential to choose the appropriate antifungal drug and to predict the. Candida albicans was the most common species isolated (33.8 %; n = 68), whereas non-albicans Candida species represented 66.2 % (n = 133) of the episodes. The species distribution and outcome of candidemia showed a difference in the crude mortality between patients infected with C. albicans (n = 30; 45.5 %) and non-albicans Candida species